Anti CD45RA Clone X148 Profile

Antigen: CD45RA
Clone: X148
Subclass IgG
Species Raised In:

Mouse

Immunogen: T cell lymphoma cells
Cross Reactivity:

Human (not yet tested in other species)

Applications: Immunohistochemistry, Western
Growth Conditions:

It is advised that cells are grown in the following medium:
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT. Ultroser G (**) can be used at 1% if the cells are not growing well.
Please note that this cell line has not been weaned out of HAT
Fusion Partner: P3/NS1/1-Ag4-1 (NS-1)
References:

Schmidt RE (1989) Non-lineage/natural killer Section report: new and previously defined cluster. In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 517-542.
Schwinzer R (1989) Cluster report: CD45/CD45R. In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 628-634.