Anti cdc27 Clone AF 3.1 Profile

Antigen: Cell division cycle protein 27 (cdc27)
Clone: AF 3.1
Subclass IgG2b
Species Raised In:

Balb/c

Immunogen: A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
Epitope:

CQLHAAESDEF

Positive Control:

HeLa cells

Cross Reactivity:

Mammalian, Xenopus laevis

Applications: Immunoprecipitation, Western
Growth Conditions:

DMEM + 5% FCS

Fusion Partner: Sp2/0Ag.14
Synonyms: Anaphase promoting complex protein 3 antibody, APC 3 antibody, Nuc2 homolog antibody
Relevance: Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis. AF 3.1 is recommended for the immunoprecipitation of active APC.
References:

Yamano H et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167 Read more