Anti DNGR-1 Clone 7H11 Profile

Antigen: Mouse DNGR-1
Clone: 7H11
Subclass IgG1
Species Raised In:

Rat

Immunogen: RBL-2H3 cells expressing mouse DNGR-1 fused to an HA epitope
Positive Control:

Rat IgG1 isotype-matched control mAbs

Cross Reactivity:

Mouse

Applications: ELISA, FACS, Immunofluorescence, Immunoprecipitation, Western
Growth Conditions:

RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME

Fusion Partner: Y3Ag 1.2.3
Relevance: Recognises mouse DNGR-1, also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
References:

Sancho et al. 2008. J Clin Invest 118:2098-110. Read more
Sancho et al. 2009. Nature. 458:899-903. Read more
Joffre et al. 2010. Eur J Immunol. 40:1255-65. Read more

Notes:

Other Applications: In Vitro and In Vivo Labelling, tetramer staining, CTL priming In Vivo, Coupling of anti-DNGR-1 to antigens and antigen targeting.