Anti DNGR-1 Clone 8F9 Profile

Antigen: Human DNGR-1
Clone: 8F9
Subclass IgG2a
Species Raised In:

Balb/c

Immunogen: RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope
Positive Control:

Mouse IgG2a isotype-matched control mAbs

Cross Reactivity:

Human

Applications: FACS
Growth Conditions:

RPMI + 10% FCS + Pen + Strep + Glutamine + 2-ME

Fusion Partner: Sp2/0Ag.14
Synonyms: CLEC9A (C-Type lectin receptor 9A)
Relevance: Recognises human DNGR-1, also known as CLEC9A, which is a highly specific marker of a human DC subset, as well as a receptor able to recognise a preformed signal exposed on necrotic cells.
References:

Sancho D et al. 2008. J Clin Invest. 118(6):2098-110. PMID: 18497879 Read more
Sancho et al. 2009. Nature. 458:899-903. PMID: 19219027 Read more
Poulin et al. 2010. J Exp Med. 207:1261-71. PMID: 20479117 Read more

Notes:

Block Fc receptor prior to staining to reduce background.