Anti HSP27 Clone ACD5 Profile

Antigen: HSP27
Clone: ACD5
Subclass IgG1
Species Raised In:

Mouse, Balb/c

Immunogen: Purified cytoplasmic oestrogen receptor fragment from human myometrium
Cross Reactivity:

Human

Applications: Immunohistochemistry, Immunoprecipitation, Paraffin embedded
Growth Conditions:

RPMI + 20% FCS, penicillin 100 (units/ml), streptomycin (100ug/ml)

Fusion Partner: P3-X63-Ag8 653
Synonyms: HSP27, heat shock protein 27
Relevance: The anti estrogen receptor (RE) antibody ACD5 (also known as D5) is capable of precipitating labelled human cytosolic RE complexes from breast tumour, fibroid, myometrial and endometrial preparations, but does not react with nuclear RE from human endometrium or cytoplasmic RE from other species tested. The antibody does not react with progesterone receptor or androgen receptor from human breast tissue, sex steroid binding globulin (SHBG) from human plasma, or rat alpha fetoprotein. The antibody is associated with a 29KDa component in RE-positive cytosols, in addition the antigen is a non hormone binding component related to cytosolic RE from breast tumour and myometrium.
References:

Coffer et al. 1985. Cancer Research. 45:3686-93. Read more
Love & King. 1994. Br J Cancer. 69:743-8. Read more
Dunn et al. 1993. J. Steroid Biochem. & Mol. Biol. 46:469-79. Read more
Zhu et al.1994. J Biol.Chem. 35: 22379-84. Read more

Notes:

Since publication of the Coffer et al. 1985 paper, work has been performed to elucidate the nature of the 29KDa protein. It is an abundant protein that had previously been discussed under three different names, p29, p24 and srp27, but it is known that they are one and the same protein and now called Heat Shock Protein 27, HSP27.