| Antigen: | Nanog |
| Species Raised In: | Rabbit |
| Immunogen: | Purified GST-Nanog 1-95 fusion protein |
| Applications: | FACS, Immunofluorescence, Western |
| Relevance: | Nanog is a transcription factor critically involved with self-renewal of undifferentiated embryonic stem cells. Human NANOG protein is a 305 amino acid protein with a conserved homeodomain motif that is localized to the nuclear component of cells. The homeodomain region facilitates DNA binding. Overexpression of Nanog in mouse embryonic stem cells causes them to self-renew in the absence of Leukemia inhibitory factor. In the absence of Nanog, mouse embryonic stem cells differentiate into visceral/parietal endoderm. Loss of Nanog function causes differentiation of mouse embryonic stem cells into other cell types. NANOG overexpression in human embryonic stem cells enables their propagation for multiple passages during which the cells remain pluripotent. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal. |
| References: | Torres et al. 2008. Nat Cell Biol. 10:194-201. PMID:18223644 Read more |
| Notes: | JT1 and JT2, same antigen used but from two different rabbits. |
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