2fTGH Cell Line

Relevance: In vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.
Description:

Panel of IFNg signalling mutant lines (10 lines plus control) each containing a different mutation in the IFN signalling pathway.

Model Type: Knockout Models
Species/Origin:

Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.

Phenotype Keywords:
Disease Keywords:
Recommended Growth Conditions:

Parental 2fTGH and mutant cell lines can be grown in DMEM with 10% FCS.

References:

McKendry, R. J. et al. 1991. Proc Natl Acad Sci U S A. 88:11455-9Read more
Pellegrini, S. J. et al. 1989. Mol Cell Biol. 9:4605-12.Read more
Rani M. R, et al. 1996. J Biol Chem. 271:22878-84Read more
Lutfalla G. 1995. EMBO J. 14:5100-8Read more

Notes:

THe 2fTGH panel is an ideal tool for investigation and comparison of the IFNg signalling pathway.
Chemical mutagenesis of the 2fTGH cell line has generated 10 IFNg signalling mutants and those that are available are:

U4C
U2A
U6A
U5A

The wild type 2FTGH human fibrosarcoma acts as a positive control together with the U5A.