| Relevance: | In vivo study of Raf-1 knockout and Ras signalling. The cells can be used to carry out drug screening toxicology studies in MEF devoid of Raf-1. |
| Description: | MEF produced from the Raf-1+/- knockout mouse. Despite the homozygous mouse dying in early embryogenesis it is possible to obtain homozygous embryos too, when they are on a mixed genetic background they survive for longer and we can get homozygotes surviving to the point when it is possible to isolate MEFs. |
| Model Type: | Mouse Embryonic Fibroblast |
| Species/Origin: | Mouse embryonic fibroblast. |
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| Disease Keywords: | |
| Recommended Growth Conditions: | DMEM+ 10% FCS+ 100U/mol penicillin/streptomycin. |
| References: | Huser et al. 2001. EMBO J. 20:1940-51. PMID: 11296227Read more |
| Notes: | See the Raf-1 knockout mouse that these cells are produced from. |
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