FLYRD18 Packaging Cell Line

Relevance: Packaging cell line enabling production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus generation.
Description:

HT1080-based packaging cell line enabling production of recombinant retroviral vectors with Moloney murine leukemia virus cores and cat endogenous virus RD114 virus envelopes.

Model Type: Viral packaging cell lines
Species/Origin:

Human; for details of production of FLYRD18 cell line see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248Read more & Takeuchi et al. 1994. Journal of Virology. 68:8001-07. PMID: 7966590Read more
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Recommended Growth Conditions:

For recommended growth and recombinant retrovirus production conditions see Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248Read more & Takeuchi et al. 1994. Journal of Virology. 68:8001-07. PMID: 7966590Read more
References:

Cosset et al. 1995. Journal of Virology. 69:7430-36. PMID: 7494248Read more
Takeuchi et al. 1994. Journal of Virology. 68:8001-07. PMID: 7966590Read more
Notes:

The FLYRD18 recombinant retroviral packaging cell line enables production of high-titer recombinant retrovirus vectors with Moloney murine leukemia virus cores, and cat endogenous virus RD114 virus envelopes.

The vectors demonstrate high resistance to the inhibitory effects of human serum/complement, increasing penetration of the vector, and making the system ideal for in vivo gene transfer.

For more high-titer retroviral gene transfer packaging systems, see also FLYA13 cell line; WE & pBabe gene transfer system