| Relevance: | Primary human atypical mortal dysplasia oral cell line, which had an extended lifespan has been immortalised by ectopic expression of hTERT. Acquisition of immortality at the dysplasia stage or oral cancer progression is consistently associated with a number of changes; namely loss of retinoic acid receptor (RAR)-beta and p16 INK4a expression, p53 mutations and activation of telomerase. |
| Description: | Normal FNB6 was obtained from a healthy volunteer; this culture senesced at 11.5 PDs, respectively. Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders in FAD+ medium. |
| Model Type: | Immortalised Primary Cell Line |
| Species/Origin: | Human |
| Phenotype Keywords: | |
| Disease Keywords: | Oral |
| Recommended Growth Conditions: | Cells were maintained on irradiated swiss 3T3 feeders in FAD+ medium (1:3 Ham’s F-l2/DMEM) with 10% FCS, insulin5ug/ml, EGF10ng/ml, transferrin5ug/ml, cholera toxin10ng/ml, hydrocortisone 400ng/ml, and adenine30ug/ml(use fresh) |
| References: | OMcGregor et al. 2002. Cancer Research. 62:4757-66. PMID:12183435 Read more
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