GFP-KSC4 keratinocyte stem cell line

Relevance: This line can be used for the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments.
Description:

Murine keratinocyte stem cell line.
Model Type:
Species/Origin:

Mouse

Phenotype Keywords:
Disease Keywords:
Recommended Growth Conditions:

The cell line grown is low calcium medium, Ham’s F12/DMEM with 10% FCS and several supplements at 32°C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 µg/ml hydrocortisone, 5 µg/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM glutamine, 1 mM pyruvate, 100 U/ml penicillin, 100 µg/ml streptomycin. Population doubling time is 22 hours. Cells are grown on plastic, coated with collagen I. Cultures are split at confluency 1:2 or 1:3, 2-3 times per week.

References:

Reichelt and Haase. 2010. Methods Mol Biol 585:59-69; PMID: 19907996 Read more
Okabe et al. 1997 FEBS Letters 407:313-9. PMID: 9175875 Read more
Höher T et al., 2011. Stem Cell Rev. PMID: 21874280. PMID: 21874280 Read more
Vollmers A et al., 2011. PMID: 21892602 Read more
Notes:

Keratinocytes were isolated from neonatal homozygous C57BL/6-Tg(CAG-EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells and are adherent.