K38 Keratinocyte Cell Line

Relevance: The prospective commercial application of the line is in the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments.
Description:

Murine keratinocyte stem cell line

Model Type:
Species/Origin:

Mouse

Phenotype Keywords:
Disease Keywords:
Recommended Growth Conditions:

The cell line grown is low calcium medium, Ham’s F12/DMEM with 10% FCS and several supplements at 32°C, 5% CO2. The supplements are: 0.18 mM adenine, 0.5 µg/ml hydrocortisone, 5 µg/ml insulin,10 -10 M cholera toxin, 10 ng/ml EGF, 2 mM glutamine, 1 mM pyruvate, 100 U/ml penicillin, 100 µg/ml streptomycin. Population doubling time is 22 hours. Cells are grown on plastic, coated with collagen I. Cultures are split at confluency 1:2 or 1:3, 2-3 times per week.

References:

Reichelt and Haase, 2010. Methods Mol Biol. 585:59-69. PMID: 19907996 Read more
Höher T et al., 2011. Stem Cell Rev. PMID: 21874280. PMID: 21874280 Read more
Vollmers A et al., 2011. PMID: 21892602 Read more
Notes:

Keratinocytes were isolated from neonatal wild-type BALB/c mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells and are adherent growers.