FGFR2 IIIb flox/flox Mouse

Gene/Protein Targets

Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb).

Relevance: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
Model Type: Knockout Models
Genetic Background: 129/C57BL/6
Zygosity: Homozygous
Phenotype Keywords:
Disease Keywords: Gastric
Production Details:

An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.

References:

De Moelooze et al. 2000. Development. 127:483-92. PMID: 10631169Read more
Grose et al. 2007. EMBO J. 26:1268-78. PMID: 17304214Read more
Notes:

For more FGFR2 IIIb knockout mouse models, see also K5-R2b-null mouse.