Cdh5(PAC)-CreERT2 Mouse

Gene/Protein Targets

Estragen receptor (ERT2) under the vascular endothelial cadherin (Cdh5(PAC)) promoter.

Relevance: Cdh5(PAC)-CreERT2 enable efficient inducible conditional recombinase expression in embryonic and adult endothelial cells (tissue-specific loxP knockout/knockin/transgene). The mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation.
Model Type: Deleter
Genetic Background: C57BL/6 or FVB/N
Zygosity: Heterozygous
Phenotype Keywords:
Disease Keywords: Angiogenesis
Production Details:

A Cdh5(PAC)-CreERT2 transgene vector, containing a genomic Cdh5(PAC) promoter fragment fused to a CreERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Cdh5(PAC)-CreERT2 transgene.

References:

Wang et al., 2010. Nature. 27:483-6. PMID: 20445537Read more
Bazigou et al., 2009 Dev Cell. 17:175-86Read more
Mahmoud et al., 2010. Circulation Research.106:1425-33Read more
Benedito et al., 2009 Cell. 137:1124-35Read more
Robson et al., 2010. Dev Dyn. 239: 2435-42Read more
Sorensen et al., 2009. Blood 113:5680-8Read more
Luna-Zurita et al., 2010 J Clin Invest. 120:3493Read more
Pitulescu et al., 2010. Nature Protocols. 5:1518-34Read more
Bréart et al., 2011. JEM. (E-Pub) Read more
Notes:

The Cdh5(PAC)-CreERT2 mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in vascular endothelial cells. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP-flanked genes in vascular endothelial cells.

Non-induced Cdh5(PAC)-CreERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Cdh5(PAC)-CreERT2 mice demonstrate high penetrance in vascular endothelial cells (95%+).
For more angiogenesis and atherosclerosis models, see also: Pdgfrb-Cre mouse; Bmx-Cre-ERT2 mouse