AhCre Mouse

Gene/Protein Targets

Cre recombinase under the CYP1A1 promoter.

Relevance: Cre under CYP1A1 promoter enabling inducible recombinase expression with administration of lipophilic xenobiotic (tissue-specific loxP knockout/knockin/transgene).
Model Type: Conditional, Deleter, Knockin Models, Knockout Models
Genetic Background: C57Bl/6
Zygosity: Homozygous
Phenotype Keywords:
Disease Keywords: Gastric
Production Details:

An AhCre transgene vector, containing the cre coding sequence under the rat CYP1A1 promoter, was injected into fertilized CBAxC57BL6 oocytes. Founder mice were backcrossed onto C57BL6 mice to establish transgenic lines. Heterozygous transgenic lines were interbred to establish homozygous AhCre mice.

References:

Ireland H et al. 2004. Gastroenterology. 126:1236-46. Read more

Notes:

The AhCre mouse exhibits sensitive, controllable Cre recombinase induction and activity upon administration of lipophylic xenobiotics (i.e. beta-napthoflavone, betaNP). The AhCre mouse is particularly useful for knockin/knockout/transgene studies of loxP flanked genes in crypt and villus epithelia of the small intestine, with Cre induction levels regulatable by dose, administration route, or timecourse.

Cre, expressed under CYP1A1 promoter, has inducible recombinase expression and is conditional to site of injection of inducing chemical.