Dll1 flox Conditonal KO Mouse

Gene/Protein Targets

Delta like-1 (Dll1)

Relevance: Conditional knockout of Dll1; in vivo studies of tissue-specific Dll1 knockout and Notch signalling. The Dll1flox mouse is unique in allowing conditional knockout and analysis of the role of Delta like-1 in late embryonic, foetal or adult tissues, and is an ideal tool in the study of Delta like-1 and Notch signalling. The Dll1flox mouse enables tissue-specific knockout of Delta like-1, a principle ligand of Notch controlling cell cell fate in multiple tissue types (neural, inner ear, haematopoietic, lymphoid, epidermal, intestinal epithelia and pancreas). Dll1flox carries loxP-flanked exons within the Dll1 locus, allowing knockout of the Dll1 gene when mated to an appropriate Cre deleter mouse.
Model Type: Conditional, Knockout Models
Genetic Background: 129/C57BL/6
Zygosity: Heterozygous
Phenotype Keywords:
Disease Keywords: Autoimmune disease
Production Details:

A Dll1 targeting vector, containing a loxP site inserted before exon 3, and a loxP-flanked resistance cassette after exon 4, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL/6 blastocyst. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Dll1 allele.

References:

Hozumi et al. 2004. Nature Immunology 5:638-644. PMID: 15146182Read more
Brooker et al. 2006. Development. 133:1277-1286. PMID: 16495313Read more