GSTP1/2-/- KO Mouse

Gene/Protein Targets

Glutathione Transferase P1 and P2 (GSTP1, GSTP2)

Relevance: In vivo study of GSTP1/2 (regulator of protein synthesis/degradation) knockout in tumourigenesis, drug metabolism and toxicity; disease model for drug metabolism and toxicity; tumourigenesis model (skin, lung) (chemically induced)
Model Type: Knockout Models
Genetic Background: 129/MF1 or 129/C57BL/6
Zygosity: Homozygous
Phenotype Keywords: Tumourigenesis
Disease Keywords: Drug Metabolism & Toxicity, Lung adenoma
Production Details:

A Gstp1/2 targeting vector, replacing all of the Gstp1 gene and exons 6 and 7 of the Gstp2 gene with a resistance cassette, was transfected into E14Tg2a.IV ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout.

References:

Henderson CJ et al. 1998. Proceedings of the National Academy of Sciences USA. 95:5275-80. PMID: 9560266Read more

Notes:

For more skin tumourigenesis models, see also Inv-a6b4 mouse.