Jag1flox mouse

Gene/Protein Targets

Jagged-1 (Jag1)

Relevance: Conditional knockout of Jag1; in vivo studies of tissue-specific Jag1 knockout and Notch signalling
Model Type: Conditional, Knockout Models
Genetic Background: 129/C57BL/6
Zygosity: Heterozygous
Phenotype Keywords:
Disease Keywords: Autoimmune disease
Production Details:

A Jag1 targeting vector, containing a loxP-flanked resistance cassette before exon 4 and a loxP site inserted after exon 5, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were transfected with a Cre expression vector to excise the resistance cassette, and injected into a C57BL/6 blastocyst. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Jag1 allele.

References:

Brooker et al. 2006. Development. 133:1277-86. PMID: 16495313Read more

Notes:

The Jag1flox mouse is unique in allowing conditional knockout and analysis of the role of Jagged-1 in late embryonic, foetal or adult tissues, and is an ideal tool in the study of Jagged-1 and Notch signalling.

The Jag1flox mouse enables tissue-specific knockout of Jagged-1, a principle ligand of Notch controlling cell cell fate in different tissue types . Jag1flox carries loxP-flanked exons within the Jag1 locus, allowing knockout of the Jag1 gene when mated to an appropriate Cre deleter mouse.

For more Notch signalling mice, see also Dll1flox mouse.